《茶叶科学》| 2022年第5期要文概览

《茶叶科学》（Chaye Kexue）

(双月刊5年期国债期货，1964年创刊）

《茶叶科学》（英文刊名：Journal of Tea Science）于1964年8月经中宣部批准创刊，刊名系朱德委员长题字，中国工程院院士陈宗懋研究员担任编委会主任，中国工程院院士刘仲华教授等专家担任编委会副主任，是茶学界唯一被中国科学引文数据收录为核心区的中文核心期刊，目前也被国内外知名数据库收录（中国知网、万方、维普、中教、EBSCO、Scopus等）5年期国债期货。

2022, Vol. 42, No.5

主管：中国科学技术协会

主办：中国茶叶学会、 中国农业科学院茶叶研究所

编辑出版：《茶叶科学》编辑部

梧州茶树种质资源的遗传多样性及亲缘关系分析

展开全文

王留彬, 黄丽蕴, 滕翠琴, 吴立赟, 成浩, 于翠平, 王丽鸳

摘要：基于SSR标记对梧州六堡镇群体种和南渡镇野生大茶树种质资源的遗传多样性和亲缘关系进行了分析,并筛选出用于该种质资源鉴别的核心分子标记5年期国债期货。研究结果如下：（1）17对SSR引物在供试材料中共扩增得到98个等位基因,每对SSR引物扩增的等位位点为3~8个,平均每个位点5.764 7个等位基因;（2）从17个SSR分子标记中筛选出8个核心标记组合即可区分每份种质资源;（3）六堡镇茶树种质资源的平均等位基因数（A）、基因型数、基因多样性（H）、多态性信息含量（PIC）分别为4.647 1、7.000 0、0.675 4、0.628 3,高于南渡镇野生大茶树种质资源,与栽培种茶树群体接近;（4）聚类分析表明,六堡镇茶树群体部分种质资源单独聚为一类,部分与云南的大叶种茶树,少量与浙江、贵州地方栽培种聚为一类;而南渡镇野生茶树种质资源单独聚为一类,仅有2个六堡镇的种质材料散落其间。综上所述,梧州茶树种质资源丰富,遗传多样性较高,研究结果为进一步开发和利用该资源奠定了基础。

关键词：SSR标记, 遗传多样性, 梧州市, 茶树

参考引用：王留彬, 黄丽蕴, 滕翠琴, 吴立赟, 成浩, 于翠平, 王丽鸳. 梧州茶树种质资源的遗传多样性及亲缘关系分析[J].茶叶科学,2022,42(5):601-609.doi:10.13305/j.cnki.jts.2022.05.004.

Genetic and Phylogenetic Analysis for Germplasm Resources of Camellia sinensis from Wuzhou City

WANG Liubin, HUANG Liyun, TENG Cuiqin, WU Liyun, CHENG Hao, YU Cuiping, WANG Liyuan

Abstract:Based on theSSR markers, the genetic diversity and genetic relationship of the germplasm resources oftea plants from Liubao town and Nandu town werefullyanalyzed inthis study. The core molecular markers for the efficient identification of these germplasm resources were successfully screened.The main results showthat: (1) 98 alleles were amplified from 17 pairs of SSR primers, and each pair of SSR primers amplified 3-8 alleles, with an average of 5.764 7 alleles per locus.(2) Totally 8 core markers were selected from 17 SSR markers to distinguish each germplasm resource.(3) The average number of alleles (4.647 1), genotypes per marker (7.000 0), genetic diversity (0.675 4), and thepolymorphic information content (0.628 3) of native tea treesin Liubao townwere higher than wild tea trees in Nandu, and close to the cultivated population. (4) Cluster analysis showsthat the majority germplasm resources from Liubao town were clustered together except for several tea plants grouped with large-leaf tea cultivars from Yunnan province and a few resources were grouped into the same clusterwithtea cultivars from Zhejiang and Guizhou provinces.The wild tea germplasm resources from Nandu town were grouped into the same cluster with twogermplasm materials from Liubao town.In conclusion, itwasshowedthattherearerich tea germplasmresources withhigh genetic diversity inWuzhou city.This study might lay a solid foundation for the further studies to develop and utilize these tea resources.

Keywords:SSR marker,genetic diversity, Wuzhou city, Camellia sinensis

Cite this Article:WANG Liubin, HUANG Liyun, TENG Cuiqin, WU Liyun, CHENG Hao, YU Cuiping, WANG Liyuan. Genetic and Phylogenetic Analysis for Germplasm Resources of Camellia sinensis from Wuzhou City[J]. Journal of Tea Science, 2022, 42(5): 601-609. doi: 10.13305/j.cnki.jts.2022.05.004.

茶树根系耐铝促生内生细菌的分离鉴定及其特性研究

武警5年期国债期货，陈楠楠， 韩梦琳，陈高，厉伟伟，张蜀香，蒋晓岚

摘要：茶树喜酸耐铝,且低浓度的铝促进茶树生长,然而其调控机理并不清晰5年期国债期货。从耐铝促生菌的角度,探究其可能的原因。以铝处理的茶树根系为材料,经分离鉴定,得到可培养的内生细菌38株,其中厚壁菌门27株,放线菌门11株。从利用1-氨基环丙烷-1-羧酸（ACC）能力、溶磷能力、产铁载体能力和分泌吲哚乙酸（Indole-3-acetic acid,IAA）能力对38株内生细菌进行了探究,结果表明,38株内生细菌都有一种以上的促生能力,其中厚壁菌FBA、FPC以及放线菌AMM、ACP032155等菌株的综合促生能力较好;38株内生细菌在1 mmol·L-1Al3+浓度下均能存活,其中放线菌AME2耐铝能力最强,在8 mmol·L-1Al3+浓度下仍能存活,说明铝能促进茶树耐铝促生菌的生长,从而间接促进茶树的生长,为选育具有显著耐铝促生能力的茶树内生细菌用于茶树的栽培育种奠定基础。

关键词：根, 内生细菌, 耐铝, 促生能力

参考引用：武警, 陈楠楠, 韩梦琳, 陈高, 厉伟伟, 张蜀香, 蒋晓岚. 茶树根系耐铝促生内生细菌的分离鉴定及其特性研究[J]. 茶叶科学, 2022, 42(5): 610-622. doi: 10.13305/j.cnki.jts.2022.05.006.

Isolation, Identification and Characterization of Aluminum-tolerant Growth-promoting Endophytic Bacteria in Tea Roots

WU Jing, CHEN Nannan, HAN Menglin, CHEN Gao, LI Weiwei, ZHANG Shuxiang, JIANG Xiaolan

Abstract:Tea plants are aluminum-tolerant plants. Low concentration of aluminum promotes the growth of tea plants, but its regulation mechanism remains unclear. In this experiment, 38 strains of culturable endophytic bacteria were isolated and identified from the roots of tea plants treated with aluminum, including 27 strains of Firmicutes and 11 strains of Actinomycetes. Plant growth promoting abilities of the isolated endophytic bacteria were explored from ACC deaminase, phosphate solubilization, siderophore and IAA production. It was found that the comprehensive plant growth promoting abilities of Firmicutes FBA, FPC and Actinomycetes AMM, ACP032155 were better. The relative activities of the 38 strains at different aluminum ion concentrations were further investigated. The results show that the 38 strains of endophytic bacteria could survive under 1 mmol·L-1Al3 +concentration, among which Actinomycetes AME2 could still survive under 8 mmol·L-1Al3+, which showed the strongest aluminum tolerance. The results show that aluminum treatment could promote the growth of aluminum-tolerant bacteria in tea plants, thus indirectly promoting the growth of tea plants. This study laid a foundation for the cultivation and breeding of endophytic bacteria of tea plants with significant aluminum tolerance and growth promotion ability.

Keywords:root, endophytic bacteria, aluminum tolerance, promoting growth abilities

Cite this Article:WU Jing, CHEN Nannan, HAN Menglin, CHEN Gao, LI Weiwei, ZHANG Shuxiang, JIANG Xiaolan. Isolation, Identification and Characterization of Aluminum-tolerant Growth-promoting Endophytic Bacteria in Tea Roots[J]. Journal of Tea Science, 2022, 42(5): 610-622. doi: 10.13305/j.cnki.jts.2022.05.006.

基于代谢组学的云南白茶与福鼎白茶化学成分比较分析

高健健5年期国债期货，陈丹，彭佳堃，吴文亮，蔡良绥，蔡亚威，田军，万云龙，孙威江，黄艳，王哲，林智，戴伟东

摘要：为了探明云南白茶与福鼎白茶的化学物质差异,以9个云南白茶茶样和6个福鼎白茶茶样为研究对象,采用基于超高效液相色谱-四极杆-静电轨道阱质谱（UHPLC-Q-Exactive/MS）的代谢组学方法并结合感官审评分析对其非挥发性化学成分进行系统研究5年期国债期货。本研究共鉴定出109个化合物,包括儿茶素类、二聚儿茶素类、黄酮糖苷类（黄酮-O -糖苷和黄酮-C -糖苷）、N -乙基-2-吡咯烷酮取代的儿茶素类、氨基酸类、酚酸类、有机酸类、生物碱类、脂质类等。偏最小二乘法判别分析和热图分析表明,云南白茶和福鼎白茶的化学成分存在较大差异,共得到46个具有组间显著性差异的化合物（P <0.05）,其中表型儿茶素类、二聚儿茶素类、部分黄酮糖苷类（山柰酚-3-半乳糖苷、槲皮素-3-葡萄糖苷等）、酚酸类、有机酸类、脂类等化合物在云南白茶中含量较高;非表型儿茶素类、部分黄酮糖苷类（槲皮素-3-半乳糖苷、杨梅素-3-半乳糖苷等）、氨基酸类、生物碱类化合物在福鼎白茶中含量相对较高,推测主要受茶树品种和干燥工艺的影响。本研究可为全面了解和认识两地白茶的化学物质基础和风味品质差异及其产地鉴别提供理论参考。

关键词：云南白茶, 福鼎白茶, 代谢组学, 液质联用, 内含成分

Comparison on Chemical Components of Yunnan and Fuding White Tea Based on Metabolomics Approach

GAO Jianjian, CHEN Dan, PENG Jiakun, WU Wenliang, CAI Liangsui, CAI Yawei, TIAN Jun, WAN Yunlong, SUN Weijiang, HUANG Yan, WANG Zhe, LIN Zhi, DAI Weidong

Abstract:In order to investigate the differences in chemical compositions between Yunnan white tea and Fuding white tea, 9 Yunnan white tea samples and 6 Fuding white tea samples were studied by ultrahigh performance liquid chromatography-quadrupole orbitrap mass spectrometer (UHPLC-Q-Exactive/MS) combined with sensory evaluation to analyze the non-volatile chemical components of white tea in two places. A total of 109 compounds were structurally identified in this study, including catechins, dimeric catechins, flavonoid glycosides (flavone/flavonol-O -glycosides and flavone/flavonol-C -glycosides), N -ethyl-2-pyrrolidinone-substituted flavan-3-ols (EPSFs), amino acids, phenolic acids, organic acids, alkaloids, lipids, et al. The partial least squares discriminant analysis and heatmap analysis show that there were distinct differences in the chemical components between Yunnan white tea and Fuding white tea. A total of 46 compounds showed significant differences between groups (P <0.05). The contents of epicatechins, dimericcatechins, flavonoid glycosides (kaempferol-3-galactoside, quercetin-3-glucoside, etc.), phenolic acids, organic acids, and lipids were relatively high in Yunnan white tea; while the contents of nonepicatechins, flavonoid glycosides (quercetin-3-galactoside, myricetin-3-galactoside, etc.), amino acids and alkaloids were relatively higher in Fuding white tea, which was speculated to be related with tea cultivars and drying processes. This study provided a theoretical basis for the understanding and recognition of the difference in the chemical substance and flavor quality of different white tea between two places, as well as the identification of white tea origins.

Keywords:Yunnan white tea, Fuding white tea, metabolomics, LC-MS, chemical composition

香叶醇生物合成相关基因 NUDX1 的进化分析

周汉琛5年期国债期货，杨霁虹，徐玉婕，吴琼，雷攀登

摘要：茶树中存在2个亚细胞定位不同的NUDX1 基因（CsNUDX1-cyto 和CsNUDX1-chlo ）,其中定位于细胞质的CsNUDX1-cyto 基因与香叶醇生物合成密切相关5年期国债期货。为探究NUDX1 基因在不同茶树品种中的序列、功能差异及其在物种间的进化,通过序列比对、基因克隆、进化树构建、代谢物分析、功能验证等分析了该基因在茶树与非茶树植物中的进化以及香叶醇积累差异。结果表明,不同茶树基因库中组装的CsNUDX1s 核苷酸序列存在差异;RT-PCR克隆显示4个阿萨姆变种和4个中国变种茶树中均有CsNUDX1-cyto 和CsNUDX1-chlo 的阳性克隆,且核苷酸序列存在差异。利用Phytozome网站数据进行序列比对及进化树构建,结果显示共有58个植物中存在CsNUDX1 同源基因;该基因在植物物种间较为保守,在低等植物藻类基因组中也存在;在单子叶禾本科植物中,除短柄草（Brachypodium distachyon ）泛基因组中存在蛋白序列匹配率大于58%的目的基因,其余均较低,尤其是在部分禾本科植物中该基因存在缺失。代谢物分析表明15个禾本科植物水稻、小麦和玉米品种鲜叶中均未检测到香叶醇的合成,而4个茶树品种嫩梢中香叶醇含量为0.87~4.12 μg·g-1。此外,茶树CsNUDX1s 基因在幼嫩叶片中有高表达;阿萨姆变种茶树佛香3号的CsNUDX1-cyto 同样具有合成香叶醇的功能。本研究表明,NUDX1 基因广泛存在植物基因组中,在茶树基因组中存在2个CsNUDX1s 基因,并与茶树叶片中香叶醇的合成相关。

关键词：香叶醇, NUDX1 基因, 进化分析, Phytozome, 茶树

参考引用：周汉琛, 杨霁虹, 徐玉婕, 吴琼, 雷攀登. 香叶醇生物合成相关基因NUDX1的进化分析[J]. 茶叶科学, 2022, 42(5): 638-648. doi: 10.13305/j.cnki.jts.2022.05.001.

Phylogenetic Analysis of NUDX1 Gene Involved in Geraniol Biosynthesis

ZHOU Hanchen, YANG Jihong, XU Yujie, WU Qiong, LEI Pandeng

Abstract: Geraniol is an acyclic monoterpene alcohol, which plays key roles in plant-environment interactions, such as pest repelling, antimicrobial activity, and pollinator attraction as well as the aroma traits for tea plants. It was reported that a cytosolic Nudix hydrolase (NUDX1) catalyzes geranyl pyrophosphate (GPP) into geranyl monophosphate (GP), followed by dephosphorylation with an endogenous phosphatase to produce geraniol. Two homologues of AtNUDX1 were found in tea genome (CsNUDX1-cyto and CsNUDX1-chlo ) with different subcellular location. Searching the homologues of AtNUDX1 on Phytozome shows that fifty-eight plant species contain the homologues of AtNUDX1 (with identities>64%). However, no homologue of AtNUDX1 was found in the genomes of grass species, with the exception of Brachypodium distachyon. We thus detected AR2000-enzymed geraniol in fresh leaves of rice, wheat, maize, and tea plants. The results show that free geraniol was undetectable in fresh leaves of rice, wheat and maize, where as young shoots of four tea cultivars had high levels of geraniol (0.87-4.12 μg·g-1). Two CsNUDX1 genes were highly expressed in young tea leaves and had a positive correlation (above 0.7) with the accumulation of geraniol. This study shows that NUDX1 s are widely present in plant genome, which are closely related to the formation of geraniol.

Keywords:geraniol, NUDX1 gene, phylogenetic analysis, Phytozome, Camellia sinensis

Cite this Article:ZHOU Hanchen, YANG Jihong, XU Yujie, WU Qiong, LEI Pandeng. Phylogenetic Analysis of NUDX1 Gene Involved in Geraniol Biosynthesis[J]. Journal of Tea Science, 2022, 42(5): 638-648. doi: 10.13305/j.cnki.jts.2022.05.001.

利用图像特征分析茶树成熟叶表型的遗传多样性

陈琪予5年期国债期货，马建强，陈杰丹，陈亮

摘要：明确我国茶树种质资源遗传多样性是对其有效利用的重要基础5年期国债期货。以国家种质杭州茶树圃中的504份茶树资源为材料,对成熟叶的18个图像特征进行统计、主成分、相关性和聚类分析,以研究基于数字图像特征的我国茶树种质资源的遗传多样性。结果表明,其变异系数和遗传多样性指数分别为15.97%和1.98。不同省份之间,平均变异系数福建最大,为16.29%,江苏最小,为10.58%;平均遗传多样性指数浙江最大,为2.01,重庆最小,为1.67。主成分分析将18个图像特征降维成4个主成分,累计贡献率达到82.63%,并从18个图像特征中筛选出了12个显著差异的图像特征。根据图像特征进行聚类分析,将504份茶树种质资源聚成6类。研究结果为以数字图像技术深入评价和利用我国茶树种质资源提供了参考依据。

关键词：茶树, 种质资源, 成熟叶片, 遗传多样性, 图像特征

参考引用：陈琪予, 马建强, 陈杰丹, 陈亮. 利用图像特征分析茶树成熟叶表型的遗传多样性[J]. 茶叶科学, 2022, 42(5): 649-660. doi: 10.13305/j.cnki.jts.2022.05.002.

Genetic Diversity of Mature Leaves of Tea Germplasms Based on Image Features

CHEN Qiyu, MA Jianqiang, CHEN Jiedan, CHEN Liang

Abstract:The genetic diversity of tea germplasm in China is an important basis for its effective utilization. In this study, the genetic diversity of tea germplasm in China was elucidated by statistical analysis, principal component analysis, correlation analysis and cluster analysis of 18 image features of mature leaves from 504 tea germplasm accessions preserved in China National Germplasm Hangzhou Tea Repository. The results show that the coefficient of variation and genetic diversity index of this population were 15.97% and 1.98, respectively. Among different provinces, the average coefficient of variation was the largest in Fujian province, which was 16.29%. The data of Jiangsu province was on the bottom, accounting for 10.58%. Zhejiang province had the highest average genetic diversity index at 2.01. The average genetic diversity index of Chongqing municipality reached the lowest point, occupying 1.67. The dimension of 18 image features were streamlined by principal component analysis and characterized into 4 principal components, with a cumulative contribution rate of 82.63%, and 12 image features were screened out from 18 image features with significant differences. According to the image features, the tea germplasms were clustered into 6 groups. The results provided a theoretical basis and reference for further exploration and utilization of tea germplasm in China.

Keywords:tea plant, germplasm, mature leaves, genetic diversity, image characteristics

Cite this Article:CHEN Qiyu, MA Jianqiang, CHEN Jiedan, CHEN Liang. Genetic Diversity of Mature Leaves of Tea Germplasms Based on Image Features[J]. Journal of Tea Science, 2022, 42(5): 649-660. doi: 10.13305/j.cnki.jts.2022.05.002.

几种改良措施对酸化茶园土壤理化性质和微生物群落结构的影响

李艳春5年期国债期货，汪航，李兆伟，叶菁，王义祥

摘要：茶园土壤酸化是制约茶树可持续生产的重要因素,通过田间小区试验分析比较几种措施对茶园土壤的改良效果,以期为茶园酸化土壤改良提供科学依据5年期国债期货。试验设置7个处理：全量化肥（常规施肥,NPK）、全量化肥+10 t•hm-2生物质炭（NPK+BC10）、有机肥替代50%化肥（OM50）、有机肥替代50%化肥+2 t•hm-2生石灰（OM50+Lime）、有机肥替代50%化肥+10 t•hm-2生物质炭（OM50+BC10）、有机肥替代50%化肥+20 t•hm-2生物质炭（OM50+BC20）、有机肥替代50%化肥+40 t•hm-2生物质炭（OM50+BC40）。连续施用2年后,对茶园土壤酸度、养分和微生物群落进行测定。与NPK相比,OM50+Lime、OM50+BC20和OM50+BC40处理土壤pH分别显著提高1.10、0.49和0.68,盐基饱和度分别显著提高114.01%、55.92%和58.62%。OM50+BC10、OM50+BC20和OM50+BC40处理的土壤有机碳含量分别比NPK处理显著增加了29.68%、41.04%和59.37%。不同处理对土壤硝态氮含量无显著影响,OM50、OM50+BC20和OM50+BC40处理的铵态氮含量比NPK处理分别显著提高了40.27%、44.77%和41.77%。NPK+BC10、OM50+BC10、OM50+BC20和OM50+BC40处理能显著提高土壤微生物活性、微生物群落物种丰富度、多样性和均一性。OM50+BC10、OM50+BC20和OM50+BC40处理显著降低了真菌/细菌比例,表明这3个处理短期内增加了红壤茶园土壤生态系统稳定性,但OM50+Lime处理的革兰氏阴性菌/革兰氏阳性菌比例显著低于对照处理,表明施用生石灰处理的土壤微生物受到的环境胁迫程度高于其他处理。总之,OM50+Lime、OM50+BC20和OM50+BC40处理对酸化茶园土壤有较好的改良效果,OM50+BC20和OM50+BC40处理对土壤微生物群落性质方面的改良效果更佳。综合考虑改良效应及成本,OM50+BC20为最佳改良方案。

关键词：茶园土壤, 酸化, 土壤微生物群落, 生物质炭, 石灰, 有机肥

参考引用：李艳春, 汪航, 李兆伟, 叶菁, 王义祥. 几种改良措施对酸化茶园土壤理化性质和微生物群落结构的影响[J]. 茶叶科学, 2022, 42(5): 661-671. doi: 10.13305/j.cnki.jts.2022.05.009.

Ameliorative Effect of Several Measures on Soil Physicochemical Properties and Microbial Community Structures in Acidified Tea Gardens

LI Yanchun, WANG Hang, LI Zhaowei, YE Jing, WANG Yixiang

Abstract:Acidification of tea garden soil is an important factor that restricts sustainable production of tea plants. Field plot experiments were conducted to study the effects of several improvement measures on acidified tea soils, which would provide scientific basis to improve the soil quality. The experiment involved several treatments: (1) pure chemical fertilizer (routine fertilization, NPK), (2) chemical fertilizer plus 10 t•hm-2biochar (NPK+BC10), (3) organic manure substituted 50% of chemical fertilizer (OM50), (4) organic manure substituted 50% of chemical fertilizer plus lime (OM50+Lime), (5) organic manure substituted 50% of chemical fertilizer plus 10 t•hm-2biochar (OM50+BC10), (6) organic manure substituted 50% of chemical fertilizer plus 20 t•hm-2biochar (OM50+BC20), and (7) organic manure substituted 50% of chemical fertilizer plus 40 t•hm-2biochar (OM50+BC40). After two years of continuous application, soil samples were taken to determine the soil acidity, the nutrient contents, and the microbial community. The microbial community structure was measured using phospholipid fatty acid (PLFA) and Biolog technology. Compared with NPK, soil pH were significantly increased by 1.10, 0.49 and 0.68 units, and base saturation were significantly increased by 114.01%, 55.92% and 58.62% in OM50+Lime, OM50+BC20, and OM50+BC40 treatments, respectively. Compared with NPK, soil organic carbon contents under OM50+BC10, OM50+BC20 and OM50+BC40 treatments were significantly increased by 29.68%, 41.04% and 59.37%, respectively. All treatments had no significant effect on soil nitrate nitrogen content, while the ammonium nitrogen contents under OM50, OM50+BC20 and OM50+BC40 treatments were significantly increased by 40.27%, 44.77% and 41.77% compared with NPK. NPK+BC10, OM50+BC10, OM50+BC20 and OM50+BC40 significantly increased soil microbial activity, species richness, diversity and homogeneity of microbial community. OM50+BC10, OM50+BC20 and OM50+BC40 treatments significantly reduced the ratios of fungi to bacteria, indicating that they increased the stability of soil ecosystem in the short term. The ratio of gram-negative bacteria to gram-positive bacteria under OM50+Lime treatment was significantly lower than that under NPK, which indicates that soil microorganism under OM50+Lime suffered the greater environmental stress than that under other treatments. In summary, OM50+Lime, OM50+BC20 and OM50+BC40 had obvious improvement effect on acidified tea garden soil, while OM50+BC20 and OM50+BC40 had better improvement effect on soil microbial community properties than OM50+Lime. Simultaneous considering ameliorative effect and cost of economy, OM50+BC20 was the best improvement scheme.

Keywords:tea garden soils, acidification, soil microbial community, biochar, lime, organic manure

Cite this Article:LI Yanchun, WANG Hang, LI Zhaowei, YE Jing, WANG Yixiang. Ameliorative Effect of Several Measures on Soil Physicochemical Properties and Microbial Community Structures in Acidified Tea Gardens[J]. Journal of Tea Science, 2022, 42(5): 661-671. doi: 10.13305/j.cnki.jts.2022.05.009.

有机管理模式对茶园土壤真菌群落结构及功能的影响

王峰5年期国债期货，陈玉真，吴志丹，尤志明，余文权，俞晓敏，杨贞标

摘要：以两种不同管理方式（常规和有机种植）和3个坡位（上、中、下坡位）表层土壤（0~20 cm）为研究对象,通过野外调查、高通量测序、功能预测结合分子生态网络分析,研究管理方式和坡位对土壤真菌群落结构、功能特征和共发生网络的影响5年期国债期货。研究结果表明,管理方式和坡位对土壤真菌α 多样性指数影响不显著,且不存在交互作用。在门水平,管理方式及不同坡位样地的优势真菌均为子囊菌门、担子菌门和被孢霉门,管理方式没有改变土壤优势真菌的种类,但影响其相对丰度。主坐标分析（PCoA）显示,管理模式导致茶园土壤真菌群落组成发生明显改变,常规管理方式下不同坡位之间的土壤真菌群落结构有显著差异（P <0.05）,而有机种植下不同坡位之间无显著差异（P >0.05）。组间群落差异分析（LEfSe）表明,37个差异物种对管理方式非常敏感,不同管理方式富集了不同的真菌类群。共现性网络分析发现,有机茶园土壤真菌网络节点数、平均聚类系数、边数、网络中心性和网络密度均明显高于常规茶园,说明其生态网络结构更为复杂。不同处理茶园土壤真菌以腐生营养型为主（66.67%~70.18%）,有机茶园土壤中内生-垃圾腐生-土壤腐生-未定义腐生真菌、木材腐生真菌和动物病原-内生-植物病原-未定义腐生真菌的丰度显著高于常规茶园,而未定义腐生真菌、植物病原真菌和动物病原-植物病原-未定义腐生真菌的丰度则显著降低。相关性分析和冗余分析结果表明,土壤全磷、有效磷、全钾、有机质、阳离子交换量和pH值是影响茶园土壤真菌群落结构的主要因子。综上所述,有机管理方式对茶园土壤真菌群落结构产生显著影响,提高了土壤有益真菌的数量（被孢霉属）,增强了土壤真菌网络稳定性和抗干扰能力,降低了病原真菌的丰度（如拟盘多毛孢属和假拟盘多毛孢属等）,具有良好的生态环境效应。

关键词：管理方式, 坡位, 真菌群落结构, 共现性网络分析, FUNGuild功能预测

参考引用：王峰, 陈玉真, 吴志丹, 尤志明, 余文权, 俞晓敏, 杨贞标. 有机管理模式对茶园土壤真菌群落结构及功能的影响[J]. 茶叶科学, 2022, 42(5): 672-688. doi: 10.13305/j.cnki.jts.2022.05.008.

Effects of Organic Management Mode on Soil Fungal Community Structure and Functions in Tea Gardens

WANG Feng, CHEN Yuzhen, WU Zhidan, YOU Zhiming, YU Wenquan, YU Xiaomin, YANG Zhenbiao

Abstract:To study the soil fungal community compositions and functional groups under different management practices and slope positions, soil samples derived from the topsoil (0-20 cm) were collected from the upper, middle, and lower slope positions of conventional and organic tea gardens. High-throughput sequencing, functional predictions and the molecular ecological network analysis were performed to investigate the community structures, functional groups and ecological network of soil fungi, respectively. The two-way ANOVA including management practices and slope positions showed that the two-way interaction had no effect on the soil fungal community α -diversity. Regardless of the management practices and the slope positions, the predominant fungal phyla were Ascomycota, Basidiomycota and Mortierellomycota. Management practices did not change the compositions of dominant fungal species, but affected their relative abundance. Principal coordinate analysis (PCoA) shows that the soil fungal community structures differed significantly among different management practices. The community structures of soil fungi at different slope positions were noticeably different under the conventional planting pattern (P <0.05), but were similar under the organic planting pattern (P >0.05). The linear discriminant analysis effect size (LEfSe) analysis shows that 37 biomarkers were very sensitive to the changes in the management, with different management practices enriching for different fungal populations. The numbers of degree, clustering, edges, degree centrality and closeness centrality of the fungal interaction network under the organic planting pattern were all higher than those under the conventional planting pattern, indicating that the fungal networks were more complex in the organic tea garden. Saprotrophic fungi were the dominant fungal group across all tea gardens (66.67%~70.18%). The effects of the management practices on soil fungal functions were obvious. Compared to those under the conventional planting pattern, the average abundance of endophyte-litter saprotroph-soil saprotroph, wood saprotroph and animal pathogen-endophyte-plant pathogen-undefined saprotroph significantly increased, but the average abundance of undefined saprotroph, plant pathogen and animal pathogen-plant pathogen-undefined saprotroph remarkably decreased. The spearman correlation analysis and the redundancy analysis (RDA) show that soil total phosphorus, available phosphorus, total potassium, organic matter, cation exchange capacity and pH were the main factors affecting the abundance and diversity of the soil fungal community. Organic planting drastically changes the structure and the compositions of the soil fungal community, enhances fungal network complexity and stability, and thus is beneficial to maintain the sustainable ecosystem in tea garden soil.

Keywords:management practices, slope position, fungal community structure, collinear network analysis, FUNGuild functional prediction

Cite this Article:WANG Feng, CHEN Yuzhen, WU Zhidan, YOU Zhiming, YU Wenquan, YU Xiaomin, YANG Zhenbiao. Effects of Organic Management Mode on Soil Fungal Community Structure and Functions in Tea Gardens[J]. Journal of Tea Science, 2022, 42(5): 672-688. doi: 10.13305/j.cnki.jts.2022.05.008.

抗小贯松村叶蝉和茶棍蓟马的茶树种质筛选及其抗性相关因素分析

孙悦5年期国债期货，吴俊，韦朝领，刘梦月，高晨曦，张灵枝，曹士先，余顺甜，金珊，孙威江

摘要：探讨不同茶树品种对小贯松村叶蝉（Matsumurasca onukii ）和茶棍蓟马（Dendrothrips minowai Priesner）的抗性,为抗虫茶树品种的选育和推广提供科学依据5年期国债期货。以11个茶树品种紫娟、水仙、梅占、白鸡冠、瑞香、奇兰、黄玫瑰、白牡丹、黄棪、大红袍、黄观音为供试材料,采用五点取样法对田间种群密度进行调查,并进行茶树植物学形态特征调查,以及叶片组织结构测定、化学成分检测,明确茶树物理性状、化学成分与抗虫性之间的相关性。结果显示,按抗小贯松村叶蝉的强弱,11个茶树品种可分为4类,第Ⅰ类：紫娟,感虫（S）;第Ⅱ类：梅占、黄棪、奇兰、水仙、白牡丹,中感（MS）;第Ⅲ类：瑞香、大红袍、黄观音、黄玫瑰,中抗（MR）;第Ⅳ类：白鸡冠,抗（R）。按抗茶棍蓟马的强弱也可将其分为4类,第Ⅰ类：黄玫瑰,感虫（S）;第Ⅱ类：瑞香、白鸡冠,中感（MS）;第Ⅲ类：黄观音、奇兰,中抗（MR）;第Ⅳ类：白牡丹、黄棪、大红袍、梅占、紫娟、水仙,抗（R）。结合相关分析显示,小贯松村叶蝉总虫口数与11个茶树品种的花青素含量、GCG含量呈极显著正相关（P <0.01）,与上角质层厚度/叶片厚度、下角质层厚度/叶片厚度呈显著正相关（P <0.05）,而与游离氨基酸总量、栅栏组织厚度、上表皮厚度、上表皮厚度/叶片厚度呈显著负相关（P <0.05）。叶长与茶棍蓟马总发生量呈显著负相关（P <0.05）,且小贯松村叶蝉的总虫口数与茶棍蓟马总虫口数显著负相关（P <0.05）。综合分析得出,大红袍茶树品种和黄观音茶树品种对小贯松村叶蝉和茶棍蓟马均存在一定抗性,是值得推广的茶树良种。调查结果还发现小贯松村叶蝉和茶棍蓟马之间存在同期交替为害茶树的现象。

关键词：茶树, 小贯松村叶蝉, 茶棍蓟马, 抗虫机理, 种质筛选

参考引用：孙悦, 吴俊, 韦朝领, 刘梦月, 高晨曦, 张灵枝, 曹士先, 余顺甜, 金珊, 孙威江. 抗小贯松村叶蝉和茶棍蓟马的茶树种质筛选及其抗性相关因素分析[J]. 茶叶科学, 2022, 42(5): 689-704. doi: 10.13305/j.cnki.jts.2022.05.007.

Screening of Tea Germplasm Resistant to Matsumurasca onukii and Dendrothrips minowai Priesner and Analysis of Resistance-related Factors

SUN Yue, WU Jun, WEI Chaoling, LIU Mengyue, GAO Chenxi, ZHANG Lingzhi, CAO Shixian, YU Shuntian, JIN Shan, SUN Weijiang

Abstract:The resistance of different tea cultivars against Matsumurasca onukii and Dendrothrips minowai Priesner was explored to provide a scientific basis for the breeding and promotion of insect resistant tea cultivars. Taking 11 tea cultivars (Zijuan, Shuixian, Meizhan, Baijiguan, Ruixiang, Qilan, Huangguanyin, Baimudan, Huangyuanyin, Dahongpao and Huangguanyin) as the test materials, the field population was investigated by five-point sampling method. Through investigation of field population density and tea plant morphological characteristics, determination of leaf tissue structure and detection of chemical composition, the relationship among physical properties, chemical components and insect resistance of tea plants was analyzed. The results show that 11 tea cultivars could be divided into 4 categories according to their resistance to M. onukii . category Ⅰ: Zijuan, insect-susceptible (S), category Ⅱ: Meizhan, Huangdan, Qilan, Shuixian, Baimudan, middle susceptible (MS), category Ⅲ: Ruixiang, Dahongpao, Huang guanyin, Huangmeigui, middle resistant (MR), category Ⅳ: Baijiguan, resistant (R). According to the strength of resistance to D. minowai Priesner, it can also be divided into 4 categories, category Ⅰ: Huangmeigui, insect-susceptible (S), category Ⅱ: Ruixiang, Baijiguan, moderate sensitivity (MS), category Ⅲ: Huang Guanyin, Qilan, middle resistant (MR), category Ⅳ: Baimudan, Huangdan, Dahongpao, Meizhan, Zijuan, Shuixian, resistant (R). Combined with correlation analysis, the total number of M. onukii was highly significantly and positively correlated with the anthocyanin content and GCG content of 11 tea cultivars (P <0.01), and significantly and positively correlated with upper cuticle thickness/leaf thickness and lower cuticle thickness/leaf thickness (P <0.05). However, it was significantly and negatively correlated with free amino acids, palisade tissue thickness, upper epidermis thickness and upper epidermis thickness/leaf thickness (P <0.05). There was a significantly negative correlation between leaf length and the total number of D. minowai Priesner (P <0.05), and the total number of M. onukii was significantly and negatively correlated with the total number of D. minowai Priesner (P <0.05). After comprehensive evaluation, the tea cultivars of Dahongpao and Huangguanyin have certain resistance to M. onukii and D. minowai Priesner, which are worthy of promotion. Meanwhile, there was a phenomenon that M. onukii and D. minowai Priesner alternately damage the tea plants in the same period.

Keywords:tea plant, Matsumurasca onukii , Dendrothrips minowai Priesner, resistance mechanism , germplasm screening

Cite this Article:SUN Yue, WU Jun, WEI Chaoling, LIU Mengyue, GAO Chenxi, ZHANG Lingzhi, CAO Shixian, YU Shuntian, JIN Shan, SUN Weijiang. Screening of Tea Germplasm Resistant to Matsumurasca onukii and Dendrothrips minowai Priesner and Analysis of Resistance-related Factors[J]. Journal of Tea Science, 2022, 42(5): 689-704. doi: 10.13305/j.cnki.jts.2022.05.007.

不同种质茶叶籽皂素含量及组成分析

陈宇宏, 高颖, 韩震, 尹军峰

摘要：茶皂素是一类在山茶科植物种子中含量丰富且具有特异生物活性的化合物,但不同种质茶叶籽中皂素含量及组成尚不清晰5年期国债期货。以浙江省同一地区采集的21个茶树品种（系）的茶叶籽为试验材料,对茶叶籽的基本特征、皂素含量及组成进行测定和分析。结果表明,不同品种（系）的茶叶籽的百粒质量、壳仁比、皂素含量均有显著性差异（P <0.05）。紫外分光光度法和高效液相色谱法（HPLC）测得21个茶树品种（系）的茶叶籽的皂素含量范围分别为30.82%~48.16%和16.93%~31.82%,其中黄观音的茶叶籽皂素含量最高。利用高效液相色谱-四极杆-静电轨道阱质谱（UHPLC-Q-Exactive/MS）同时检测出68种茶叶籽皂素单体,其中Theasaponin E1的峰相对强度最大。正交偏最小二乘法判别分析（OPLS-DA）显示,21个品种（系）的茶叶籽以树型为依据被明显地划分为2组,组间差异单体物质有21种。结合相关性分析,高积累的Theasaponin E12、Camelliasaponin B1/B2、Theasaponin A5/A6、Camelliasaponin C1/C2和Assamsaponin G可能是灌木型种质资源有别于小乔木型、乔木型种质资源的重要特征物质。研究结果为生产茶叶籽皂素原料的选择和高值化利用奠定了基础。

关键词：茶叶籽, 皂素, 单体, 超高效液相色谱-高分辨质谱

参考引用：陈宇宏, 高颖, 韩震, 尹军峰. 不同种质茶叶籽皂素含量及组成分析[J]. 茶叶科学, 2022, 42(5): 705-716. doi: 10.13305/j.cnki.jts.2022.05.005.

Analysis of the Saponin Contents and Composition in Tea Seeds of Different Germplasms

CHEN Yuhong, GAO Ying, HAN Zhen, YIN Junfeng

Abstract:Tea saponins are abundant in the seeds of Camellia sinensis with specific biological activities. Their contents and composition in tea seeds of different germplasms remain unclear. In this study, seeds from 21 tea germplasms were collected from the same region of Zhejiang and used as experimental materials to determine the basic characteristics, saponin contents and composition. Correlation analysis between germplasm and saponin composition was also performed. The results show that the seed weight, shell kernel ratio and saponin content of different tea seeds were significantly different (P <0.05). The saponin content detected by UV spectral method and HPLC ranged from 30.82% to 48.16% and 16.93% to 31.82%, respectively. ‘Huangguanyin' had the highest saponin content in tea seeds. Totally 68 tea seed saponin monomers were detected simultaneously using ultrahigh performance liquid chromatography-quadrupole orbitrap mass spectrometer（UHPLC-Q-Exactive/MS）. Theasaponin E1 had the highest relative intensity. Orthogonal partial least squares discriminant analysis (OPLS-DA) shows that the 21 tea seed germplasms can be distinguished into 2 groups according to tree type with 21 different Camelliasaponin B1/B2, Theasaponin A5/A6, Camelliasaponin C1/C2 and Assamsaponin G were the most significant characteristic substances of semi-tree form and arbor form resources. The results of the study laid the foundation for the selection and value-added utilization of tea seed saponins.

Keywords: tea seed, saponin, monomer, UHPLC-Q-Exactive/MS

Cite this Article:CHEN Yuhong, GAO Ying, HAN Zhen, YIN Junfeng. Analysis of the Saponin Contents and Composition in Tea Seeds of Different Germplasms[J]. Journal of Tea Science, 2022, 42(5): 705-716. doi: 10.13305/j.cnki.jts.2022.05.005.

控温渥堆对黑毛茶香气品质的影响

陈慧5年期国债期货，杨丽玲，陈金华，黄建安，龚雨顺，李适

摘要：对传统渥堆与45℃、50℃控温渥堆的黑毛茶进行感官品质评价,采用顶空固相微萃取-气相色谱-质谱联用技术（HS-SPME-GC-MS）结合主成分分析法（PCA）和偏最小二乘判别分析法（PLS-DA）对挥发性成分进行比较分析5年期国债期货。结果表明,与传统渥堆和50℃控温渥堆相比,45℃控温渥堆黑毛茶香气的愉悦感、纯正度和浓度更好,杂异气味少。在传统及控温渥堆中共获得24种关键性差异挥发性成分,其中α -柏木烯、新植二烯、橄榄醇、δ -杜松醇、香芹酚、2,6-二叔丁基对甲酚、(Z )-7-十六碳烯醛、反式-β -紫罗兰酮等关键性差异挥发性成分在控温渥堆中的相对含量显著高于传统渥堆。研究结果可为改善黑毛茶香气品质提供参考。

关键词：黑毛茶, 挥发性成分, 控温渥堆, 气相色谱-质谱联用技术

Effect of Temperature-controlled Pile-fermentation on Aroma Quality of Primary Dark Tea

CHEN Hui, YANG Liling, CHEN Jinhua, HUANG Jian'an, GONG Yushun, LI Shi

Abstract:Sensory quality evaluation of the primary dark tea under traditional, 45℃, 50℃ controlled pile-fermentation was performed. Meanwhile, volatile components were compared and analyzed by headspace solid phase microextraction and gas chromatography-mass spectrometry (HS-SPME-GC-MS) combined with principal component analysis (PCA) and partial least-squares discrimination analysis (PLS-DA). The results show that compared with traditional and 50℃-controlled pile-fermentation, the primary dark tea under 45℃-controlled pile-fermentation presented better aroma pleasure, purity and concentration. Furthermore, it appeared less impurity odor. A total of 24 key different volatile components were obtained from traditional and temperature-controlled pile-fermentation. The relative contents of α -cedrene, neophytadiene, olivetol, δ -cadinol, carvacrol, 2,6-di-tert-butyl-p-cresol, (Z )-7-hexadecenal, trans-β -ionone were significantly higher under the temperature-controlled pile-fermentation than those under traditional pile-fermentation. The research results could provide basis and reference for improving aroma quality of primary dark tea.

Keywords:primary dark tea, volatile components, temperature-controlled pile-fermentation, GC-MS

壳聚糖复合 β -乳球蛋白负载EGCG纳米粒的制备及其对糖尿病小鼠血糖的影响

陈珂, 王苑竹, 杨晓颖, 张冬英, 朱强强

摘要：表没食子儿茶素没食子酸酯（EGCG）作为茶叶中主要生物活性成分,具有良好的生理功能,但低稳定性使其容易被氧化降解,生物利用率低5年期国债期货。利用羧甲基壳聚糖（CMC）、壳聚糖盐酸盐（CHC）、β -乳球蛋白（β -LG）作为壁材,制备壳聚糖复合β -乳球蛋白负载EGCG纳米粒,通过透射电镜、结构表征（粒径、Zeta电位测定）对颗粒微观形态进行观察,利用高效液相色谱仪对颗粒包埋率、模拟胃肠液释放率进行测定,最后建立糖尿病小鼠模型,探究包埋后颗粒的降血糖活性。结果表明,CS-β -LG-EGCG纳米粒结构完整、粒径10~100 nm、粒子分散;包埋率大于50%,且在肠液和胃液中具有缓释作用;CS-β -LG-EGCG纳米粒与胰岛素无拮抗作用,与未包埋的EGCG相比,包埋后颗粒具备的缓释作用可减缓血糖的回升。

关键词：EGCG, 壳聚糖, β -乳球蛋白, 模拟胃液, 糖尿病

参考引用：陈珂, 王苑竹, 杨晓颖, 张冬英, 朱强强. 壳聚糖复合 β -乳球蛋白负载EGCG纳米粒的制备及其对糖尿病小鼠血糖的影响[J]. 茶叶科学, 2022, 42(5): 731-739. doi: 10.13305/j.cnki.jts.2022.05.003.

Preparation of Nanoparticules with Chitosan Complexed β -lactoglobulin Loaded EGCG and their Effects on Blood Glucose in Diabetic Mice

CHEN Ke, WANG Yuanzhu, YANG Xiaoying, ZHANG Dongying, ZHU Qiangqiang

Abstract:As the main bioactive component in tea, EGCG has abundant physiological functions. However, its low stability makes it easy to be degraded and has low bioavailability. In this experiment, carboxymethyl chitosan (CMC), chitosan hydrochloride (CHC), and β -lactoglobulin (β -LG) were used as wall materials to encapsulate EGCG. The microscopic morphology of particles were observed through electron microscope microscopic morphological observation, structural characterization (measurement of particle size, Zeta potential). The entrapment efficiency and simulated gastrointestinal fluid release rate were determined by HPLC. Finally, the activities of nanoparticles were explored by measuring the effect of nanoparticles on blood glucose in diabetic mice. The result shows that the prepared CS-β -LG-EGCG nanoparticles had complete structures, particle size of 10-100 nm, and certain dispersibilities. The entrapment efficiency was greater than 50%, and it had a slow-release effect in intestinal and gastric juices. CS-β -LG-EGCG nanoparticles had no antagonistic effect with insulin. Compared with uncoated EGCG, the sustained-release effect of the particles can slow down the recovery of blood glucose.

Keywords:EGCG, chitosan, β -lactoglobulin, simulated gastric juice, diabetes

Cite this Article:CHEN Ke, WANG Yuanzhu, YANG Xiaoying, ZHANG Dongying, ZHU Qiangqiang. Preparation of Nanoparticules with Chitosan Complexed β -lactoglobulin Loaded EGCG and their Effects on Blood Glucose in Diabetic Mice[J]. Journal of Tea Science, 2022, 42(5): 731-739. doi: 10.13305/j.cnki.jts.2022.05.003.

中国与RCEP成员国茶叶贸易竞争性与互补性分析

李峥, 刘锭, 霍增辉, 陈富桥

摘要：区域全面经济伙伴关系协定（Regional Comprehensive Economic Partnership,RCEP）的签署为区域内贸易自由化提供了便利,考察RCEP对中国茶叶进出口贸易的影响具有重要的现实意义5年期国债期货。基于2011—2020年中国与RCEP其他成员国茶叶进出口贸易数据,在分析各国进出口现状基础上,测算显示性比较优势指数（RCA）、出口相似度指数（ESI）、贸易互补性指数（TCI）、贸易结合度指数（TI）、产业内贸易指数（GL）等指标,考察双边茶叶贸易的竞争性与互补性。结果表明,中国茶叶出口与越南、印度尼西亚等国的竞争性较为突出,茶叶出口长期具有比较优势,绿茶出口比较优势明显,而大包装红茶出口的比较优势落后于印度尼西亚和越南;中国茶叶出口与日本、泰国、新加坡、韩国等茶叶出口相似度较高,在出口结构上具有较强的竞争性;中国与新西兰、文莱、澳大利亚等国的茶叶贸易互补性较高,与印度尼西亚、新西兰和越南等双边茶叶贸易在个别年份以产业内贸易为主,与澳大利亚、缅甸等国双边茶叶贸易以产业间贸易为主。最后提出在RCEP框架下我国拓展潜在市场、应对技术贸易壁垒及优化出口结构等方面的政策建议。

关键词：区域全面经济伙伴关系协定, 茶叶贸易, 竞争性, 互补性

参考引用：李峥, 刘锭, 霍增辉, 陈富桥. 中国与RCEP成员国茶叶贸易竞争性与互补性分析[J]. 茶叶科学, 2022, 42(5): 740-752. doi: 10.13305/j.cnki.jts.2022.05.010.

Analysis on the Competitiveness and Complementarity of Tea Trade between China and RCEP Members

LI Zheng, LIU Ding, HUO Zenghui, CHEN Fuqiao

Abstract:The signing of RCEP had facilitated intra-regional trade liberalization. It is of great practical significance to investigate the impact of RCEP on China's tea import and export trade. Based on the tea trade data of China and other RCEP members from 2011 to 2020, combining the analysis of the current situation of intra-regional import and export trade, this study calculated several indicators such as revealed comparative advantage index (RCA), export similarity index (ESI), trade complementarity index (TCI), trade intensity index (TI) and intra-industry trade index (GL) to measure the competitiveness and complementarity of bilateral tea trade. The results indicate that the tea export competitions between China, Vietnam and Indonesia were more prominent. China's tea export had long-term comparative advantages, and the comparative advantages of green tea export were obvious, while the comparative advantages of China's export of large packaged black tea lag behind Indonesia and Vietnam. China's tea export was highly similar to Japan, Thailand, Singapore and South Korea, and had strong competitiveness in export structure. In addition, the tea trade between China and New Zealand, Brunei, Australia was highly complementary. China's tea trade with Indonesia, New Zealand and Vietnam was mainly intra-industry trade in some years, while with Australia and Myanmar was mainly inter-industry trade. Finally, under the framework of RCEP agreement, strategies including expanding potential markets, dealing with technical barriers to trade and optimizing export structure were proposed.

Keywords:RCEP, tea trade, competitiveness, complementarity

Cite this Article:LI Zheng, LIU Ding, HUO Zenghui, CHEN Fuqiao. Analysis on the Competitiveness and Complementarity of Tea Trade between China and RCEP Members[J]. Journal of Tea Science, 2022, 42(5): 740-752. doi: 10.13305/j.cnki.jts.2022.05.010.

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